User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objective== | ||
* | * Testing for HRP (UV/Vis) | ||
# Spin down HRP GNPs and resuspend pellets for second time | |||
* Work on paper and presentation | |||
* Tomorrow: Kinetics | |||
==Procedure== | |||
#Run UV-Vis on HRP conjugate pellets and supernatants | |||
#AA of samples to ensure that gold is present | |||
##Calibration of [[User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook|Javier's Notebook]] used | |||
==OPD UV/Vis== | |||
[[Image:Screen Shot 2014-04-13 at 7.40.20 PM.png]] | |||
==Notes== | |||
* Gamma method #2 did not work | |||
* Took AA of gamma supernatant | |||
** No gold, so the gold never attached or it attached and then fell off | |||
# Differences between this batch and the other batch | |||
## More gold nanoparticles in this batch than previous | |||
## This was after second spin | |||
### This could mean there was more time for them to fall into solution | |||
### The time difference alone between attachment could show that the particles are not staying attached. | |||
Latest revision as of 23:55, 26 September 2017
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Objective
Procedure
OPD UV/VisNotes
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