User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/16: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Entry title==
==Objective==
* Insert content here...
1. Test Spun down proteins for activity
2. Make conjugates at a much higher concentration
 
==Procedure==
-Testing Myoglobin and Hemoglobin
#Use Ocean optics
##Sample of centrifuged solution
##10mM Degassed Sodium Dithionite
 
-Making new conjugates for the alpha method
#25mL of AuNP solution
#25mL of protein (myoglobin and hemoglobin) in 50mM Tris buffer
#Placed in fridge until testing
 
==Data==
[[Image:MyoglobinDTT.png+DML|300px]]
This is the result of the centrifuged Myoglobin solution
It shows Myoglobin activity which means spinning at high speeds does not denature protein or break them apart
 
The same result was found for the Hemoglobin solution
 




Latest revision as of 23:55, 26 September 2017

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Objective

1. Test Spun down proteins for activity 2. Make conjugates at a much higher concentration

Procedure

-Testing Myoglobin and Hemoglobin

  1. Use Ocean optics
    1. Sample of centrifuged solution
    2. 10mM Degassed Sodium Dithionite

-Making new conjugates for the alpha method

  1. 25mL of AuNP solution
  2. 25mL of protein (myoglobin and hemoglobin) in 50mM Tris buffer
  3. Placed in fridge until testing

Data

This is the result of the centrifuged Myoglobin solution It shows Myoglobin activity which means spinning at high speeds does not denature protein or break them apart

The same result was found for the Hemoglobin solution



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