User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22

From OpenWetWare

< User:Daniel-Mario Larco | Notebook | AU Biodesign Lab - 09/03/2013 | 2014 | 04(Difference between revisions)
Jump to: navigation, search
(Autocreate 2014/04/22 Entry for User:Daniel-Mario_Larco/Notebook/AU_Biodesign_Lab_-_09/03/2013)
Current revision (05:45, 25 April 2014) (view source)
(Data)
 
(2 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objective==
-
* Insert content here...
+
1. Testing Pellets and Supernatants of concentrated solutions
 +
 
 +
Notes
 +
Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm
 +
The pellet was much larger than before. the supernatant was removed and store for testing
 +
 
 +
==Procedure==
 +
#The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
 +
#Tris buffer and Sodium DIthionite in Tris buffer were degassed
 +
#Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)
 +
 
 +
==Data==
 +
[[Image:Alpha Hemoglobin Pellet.png+DML|500px]]
 +
 
 +
The results for the hemoglobin pellet after correction
 +
 
 +
 
 +
[[Image:Alpha Myoglobin Pellet.png+DML|500px]]
 +
 
 +
The results for the myoglobin pellet after correction
 +
 
 +
 
 +
[[Image:AuNPDTT.png|500px]]
 +
 
 +
AuNP solution run with Sodium Dithionite to correct protein solutions
 +
 
 +
 
 +
 

Current revision

Project name Main project page
Previous entry      Next entry

Objective

1. Testing Pellets and Supernatants of concentrated solutions

Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing

Procedure

  1. The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
  2. Tris buffer and Sodium DIthionite in Tris buffer were degassed
  3. Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)

Data

The results for the hemoglobin pellet after correction


The results for the myoglobin pellet after correction


AuNP solution run with Sodium Dithionite to correct protein solutions




Personal tools