User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22: Difference between revisions
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== | ==Objective== | ||
1. Testing Pellets and Supernatants of concentrated solutions | |||
Notes | |||
Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm | |||
The pellet was much larger than before. the supernatant was removed and store for testing | |||
==Procedure== | |||
#The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm | |||
#Tris buffer and Sodium DIthionite in Tris buffer were degassed | |||
#Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd) | |||
==Data== | |||
[[Image:Alpha Hemoglobin Pellet.png+DML|300px]] | |||
The results for the hemoglobin pellet after correction | |||
[[Image:Alpha Myoglobin Pellet.png+DML|300px]] | |||
The results for the myoglobin pellet after correction | |||
[[Image:AuNPDTT.png|300px]] | |||
AuNP solution run with Sodium Dithionite to correct protein solutions | |||
Revision as of 02:44, 25 April 2014
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Objective1. Testing Pellets and Supernatants of concentrated solutions Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing Procedure
Data
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