User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objective== | ||
1. Testing Pellets and Supernatants of concentrated solutions | |||
Notes | |||
Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm | |||
The pellet was much larger than before. the supernatant was removed and store for testing | |||
==Procedure== | |||
#The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm | |||
#Tris buffer and Sodium DIthionite in Tris buffer were degassed | |||
#Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd) | |||
==Data== | |||
[[Image:Alpha Hemoglobin Pellet.png+DML|500px]] | |||
The results for the hemoglobin pellet after correction | |||
[[Image:Alpha Myoglobin Pellet.png+DML|500px]] | |||
The results for the myoglobin pellet after correction | |||
[[Image:AuNPDTT.png|500px]] | |||
AuNP solution run with Sodium Dithionite to correct protein solutions | |||
Latest revision as of 23:55, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objective1. Testing Pellets and Supernatants of concentrated solutions Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing Procedure
DataThe results for the hemoglobin pellet after correction The results for the myoglobin pellet after correction AuNP solution run with Sodium Dithionite to correct protein solutions
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