User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/04/22

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Objective

1. Testing Pellets and Supernatants of concentrated solutions

Notes Thursday 04/17, I ran the solutions in the centrifuge for 3 hours at 18,000 rpm The pellet was much larger than before. the supernatant was removed and store for testing

Procedure

  1. The solutions were placed in the centrifuge for a second spin down for 3 hours at 1,8000 rpm
  2. Tris buffer and Sodium DIthionite in Tris buffer were degassed
  3. Ocean Optics spectroscopy of pellets and supernatants (1st and 2nd)

Data

The results for the hemoglobin pellet after correction


The results for the myoglobin pellet after correction


AuNP solution run with Sodium Dithionite to correct protein solutions




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