User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/10: Difference between revisions
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#A small amount of 5mM Tris buffer was used to collect remaining traces of cells from the centrifuge tubes | #A small amount of 5mM Tris buffer was used to collect remaining traces of cells from the centrifuge tubes | ||
#The pellet was then sonified | #The pellet was then sonified | ||
##30 seconds | ##30 seconds sonification followed by 30 seconds on ice until the pellet was loose and liquid | ||
#Once liquefied, the black supernatant was put back in the tubes and the solution was centrifuged at 20,000rpm four 3 hours at 4C | #Once liquefied, the black supernatant was put back in the tubes and the solution was centrifuged at 20,000rpm four 3 hours at 4C | ||
##The tubes were spun for an additional 30 minutes under the same conditions in order to prepare the materials for the next step | ##The tubes were spun for an additional 30 minutes under the same conditions in order to prepare the materials for the next step |
Revision as of 07:30, 18 July 2014
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Cell and Protein Extraction
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