User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/14: Difference between revisions

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==SDS-PAGE==
==SDS-PAGE==
#Our protein solution was collected from the cold room and transferred into a 50mL falcon tube
#Our protein solution was collected from the cold room and transferred into a 50mL falcon tube labeled Purified Hb pH7, 7/14/14
#We will run a gel with the solution and past solution to check purity
#We will run a gel with the solution and past solution to check purity
#The gel will be set up in the following order
#The gel will be set up in the following order
##Myoglobin and BSA ladder
##Myoglobin and BSA ladder
##Empty
##Empty
##
##Protein in pH8.3 tris (solution that was injected into Q-Sepharose column)
##
##Elute during injection of Protein in pH8.3 tris into Q-Sepharose column
##
##Elute during cleaning Q-Sepharose column containing Protein in pH8.3 tris with 1M NaCl
##
##Elute from SP-Sepharose column in phosphate buffer pH7.2 during injection (from fractions collected from Q-Sepharose column)
##
##Empty
##
##1/10 dilution of Ammonium sulfate treatment pellet dissolved in 50mM Tris
##
##1/10 dilution of purified protein from dialysis over the weekend
##
##1/100 dilution of purified protein from dialysis over the weekend
##
##Empty
##
##Myoglobin and BSA ladder
##
 
##


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Revision as of 06:27, 14 July 2014

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SDS-PAGE

  1. Our protein solution was collected from the cold room and transferred into a 50mL falcon tube labeled Purified Hb pH7, 7/14/14
  2. We will run a gel with the solution and past solution to check purity
  3. The gel will be set up in the following order
    1. Myoglobin and BSA ladder
    2. Empty
    3. Protein in pH8.3 tris (solution that was injected into Q-Sepharose column)
    4. Elute during injection of Protein in pH8.3 tris into Q-Sepharose column
    5. Elute during cleaning Q-Sepharose column containing Protein in pH8.3 tris with 1M NaCl
    6. Elute from SP-Sepharose column in phosphate buffer pH7.2 during injection (from fractions collected from Q-Sepharose column)
    7. Empty
    8. 1/10 dilution of Ammonium sulfate treatment pellet dissolved in 50mM Tris
    9. 1/10 dilution of purified protein from dialysis over the weekend
    10. 1/100 dilution of purified protein from dialysis over the weekend
    11. Empty
    12. Myoglobin and BSA ladder



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