User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/24: Difference between revisions
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#A 1/100th dilution of this solution was prepared and a UV-Vis spectra was taken of it | #A 1/100th dilution of this solution was prepared and a UV-Vis spectra was taken of it | ||
#Using Beer's Law the concentration of protein was calculated | #Using Beer's Law the concentration of protein was calculated | ||
##Molar Absorptivity at 415nm: 128,000 | |||
##Absorbance at 415nm : 0.036 | |||
##The concentration of protein (not in diluted sample) was calculated to be 28.125uM | ##The concentration of protein (not in diluted sample) was calculated to be 28.125uM | ||
##This corresponds to 2.53125 * 10^-7 moles of Hemoglobin | ##This corresponds to 2.53125 * 10^-7 moles of Hemoglobin |
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