User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#Run a 0-300mM NaCl in Tris gradient over 30 minutes and collect fractions | #Run a 0-300mM NaCl in Tris gradient over 30 minutes and collect fractions | ||
#Combine desired fractions with added 20mM pH 8.3 Tris buffer | #Combine desired fractions with added 20mM pH 8.3 Tris buffer | ||
#Concentrate solution in centrifuge | |||
#Inject solution into Q-Sepharose column | |||
##Collect elute during injection | |||
#Run a 0-300mM NaCl in Tris gradient over 60 minutes and collect fractions | |||
#Combine desired fractions with added 10mM pH 7.2 Phosphate buffer | |||
##Two sets of fractions were collected in separate tubes corresponding to two peaks | |||
#Concentrate solution in centrifuge | #Concentrate solution in centrifuge | ||
Latest revision as of 00:09, 27 September 2017
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Preparing sample
Purification
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