User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/29: Difference between revisions
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== | ==Preparing sample== | ||
#Concentrate protein solution | |||
##Place in centrifugal device at 3000g, 4C for 1 hour and 15 minutes | |||
##Centrifuge again with pH 8.3 20mM Tris added for a total volume of 60mL | |||
##Centrifuge until the solution is reduced to ~10mL | |||
==Purification== | |||
#Inject solution into Q-Sepharose column | |||
##Collect elute during injection | |||
#Run a 0-300mM NaCl in Tris gradient over 30 minutes and collect fractions | |||
#Combine desired fractions with added 20mM pH 8.3 Tris buffer | |||
#Concentrate solution in centrifuge | |||
Revision as of 06:16, 29 July 2014
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Preparing sample
Purification
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