User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==PCR amplification of chromatin remodeling==
* Insert content here...


{| {{table}} border="1" cellspacing="3"
<!-- Editing: the coding for this table is a bit more advanced. -->
<!-- valign="top" aligns all the text in the first row to the top.  -->
<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
<!-- The next two "rowspan=7" cells span  all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
<!-- &nbsp; is ASCII code for an invisible space. -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. ANM5 = 1911, <br>2. MYB = 159,1764 <br>3. Carm1 = 1827<br>4. SETD7 = 459,642<br>5. KMT2E = 1176,4401<br>6. SETD1A = 420,4704<br>
| rowspan="7" | [[Image:http://openwetware.org/images/4/48/Screen_Shot_2015-05-14_at_1.14.53_PM.png|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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| DNA(plasmid) || 1.0 μL
|-
| 1X GoTaq || 12.5
|-
| F primer || 1.0
|-
| R Primer || 1.0
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| dH<sub>2</sub>O || 9.5
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| &nbsp; || 25 μL
|}
==Transforming P300 plasmids==
[http://openwetware.org/wiki/Haynes:TransformationPlasmids#Traditional_Method:_Chemically_competent_cells_.2B_plasmid_DNA Transformation protocol]
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''Expected'''
| align="center" style="background:#f0f0f0;"|'''Observed'''
| align="center" style="background:#f0f0f0;"|'''colonies'''
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| PBS P300||Growth||None||0.00
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| NHA P300||Growth||None||0.00
|-
| CHA P300||Growth||None||0.00
|}
Plasmids were sent on filter paper and is possible did not remain intact during transit.


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Latest revision as of 01:02, 27 September 2017

Project name Main project page
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PCR amplification of chromatin remodeling

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Transforming P300 plasmids

Transformation protocol

Sample ID Expected Observed colonies
PBS P300 Growth None 0.00
NHA P300 Growth None 0.00
CHA P300 Growth None 0.00

Plasmids were sent on filter paper and is possible did not remain intact during transit.


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