User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/30: Difference between revisions
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(Autocreate 2015/06/30 Entry for User:Daniel_A._Vargas/Notebook/General_lab_notebook) |
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== | ==Gel electrophoresis of PCR amp== | ||
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
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| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. ANM5 = 1911, <br>2. MYB = 159,1764 <br>3. Carm1 = 1827<br>4. SETD7 = 459,642<br>5. KMT2E = 1176,4401<br>6. SETD1A = 420,4704<br> | |||
| rowspan="7" | [[Image:http://openwetware.org/images/4/48/Screen_Shot_2015-05-14_at_1.14.53_PM.png|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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| DNA(plasmid) || 1.0 μL | |||
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| 1X GoTaq || 12.5 | |||
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| F primer || 1.0 | |||
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| R Primer || 1.0 | |||
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| dH<sub>2</sub>O || 9.5 | |||
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| || 25 μL | |||
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Revision as of 10:28, 30 June 2015
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Gel electrophoresis of PCR amp
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