User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2015/08/03 Entry for User:Daniel_A._Vargas/Notebook/General_lab_notebook)
 
(fix raw html notebook nav)
 
(5 intermediate revisions by one other user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Open Chromatin</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Quality Check of PCR and RED==
* Insert content here...
MV10 digest
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Reagent'''
| align="center" style="background:#f0f0f0;"|'''Volume'''
|-
| MV10 |||15.00
|-
| Xba1 |||1.00
|-
| MBN|||1.00
|-
| 10X FD buffer |||3.00
|-
| dH<sub>2</sub>O||| 10.00
|-
| Total  |||30.00
|-
Run at 37<sup>o</sup>C for 15 min
 
{| {{table}} border="1" cellspacing="3"
<!-- Editing: the coding for this table is a bit more advanced. -->
<!-- valign="top" aligns all the text in the first row to the top.  -->
<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
<!-- The next two "rowspan=7" cells span  all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
<!-- &nbsp; is ASCII code for an invisible space. -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. MV10 = 5191, <br>2. ATF2 = 906 <br>3. ATF2 neg ctrl = 0<br>4. Gal4db/mCherry = 1170<br>5. Gal4db/mCherry = 0<br>
| rowspan="7" | [[image: QC_of_pcr_red.jpg|200px|Damaged Gel]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
| DNA || 3.0 μL
|-
| 6X BioLabs purple dye || 3.0 μL
|-
| dH<sub>2</sub>O || 4.0 μL
|-
| &nbsp; || 10 μL
|}
 
Qiagen clean-up Results
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Plasmid'''
| align="center" style="background:#f0f0f0;"|'''Conc. (ng/µL)'''
| align="center" style="background:#f0f0f0;"|'''St Dev (ng/µL)'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
|-
| MV10 ||46.37||1.13||1.75
|-
| ATF2 ||64.350||2.15||1.90
|-
| Gal4DB/mCherry||50.873||1.13||1.93
|-




Latest revision as of 01:04, 27 September 2017

Open Chromatin Main project page
Previous entry      Next entry

Quality Check of PCR and RED

MV10 digest

Run at 37oC for 15 min
Reagent Volume
MV10 15.00
Xba1 1.00
MBN 1.00
10X FD buffer 3.00
dH2O 10.00
Total 30.00
Reagent Volume Expected:
1. MV10 = 5191,
2. ATF2 = 906
3. ATF2 neg ctrl = 0
4. Gal4db/mCherry = 1170
5. Gal4db/mCherry = 0
 Damaged Gel
10 μL/lane; 1% agarose; Ladder
DNA 3.0 μL
6X BioLabs purple dye 3.0 μL
dH2O 4.0 μL
  10 μL

Qiagen clean-up Results

Plasmid Conc. (ng/µL) St Dev (ng/µL) 260/280
MV10 46.37 1.13 1.75
ATF2 64.350 2.15 1.90
Gal4DB/mCherry 50.873 1.13 1.93


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Daniel_A._Vargas/Notebook/General_lab_notebook/2015/08/03&oldid=1017069"