User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/04: Difference between revisions
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| Reagent | | Reagent || Rxn1 || Rxn2 | ||
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| 30 fmol/μL MV10 || 2.0 || 2.0 | | 30 fmol/μL MV10 || 2.0 || 2.0 |
Revision as of 12:22, 4 August 2015
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ASSEMBLY of MV10, ATF2, and Gal4DB/mCherry
LCR Assembly Prep the Bridge Oligos
Overview: Mix double-stranded DNA fragments, treat with PNK to add phosphate groups
Set up the following in PCR tubes:
Master Mix (in 1.5 mL tube)
Add master mix into 10 μL PNK DNA
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