User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/04: Difference between revisions
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LCR Reactions | LCR Reactions | ||
# MV10 + Gal4DB-mCh + ATF2 | # MV10 + Gal4DB-mCh + ATF2 | ||
# MV10 | # MV10 (neg. ctrl.) | ||
* Using the entire PNK DNA reaction, add other components (into the same PCR tubes) as described in the table below | * Using the entire PNK DNA reaction, add other components (into the same PCR tubes) as described in the table below |
Revision as of 13:05, 4 August 2015
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ASSEMBLY of MV10, ATF2, and Gal4DB/mCherry
LCR Assembly Prep the Bridge Oligos
Overview: Mix double-stranded DNA fragments, treat with PNK to add phosphate groups
Set up the following in PCR tubes:
Master Mix (in 1.5 mL tube)
Add master mix into 10 μL PNK DNA
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