User:Daniel Goodman/Notebook/Cluzel/2010/02/24: Difference between revisions
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====Deposition Protocol==== | ====Deposition Protocol==== | ||
# place on glass slide: (try this first) | # place on glass slide: (try this first) | ||
## dry cells for 1, 5, 10 min | ## dry cells for 1, 5, 10 min in middle of glass slide | ||
* 2 replicates per wait time | |||
* 6 glass slides total | |||
* cover top of agarose w/ cover slip to simulate similar pressure in device | |||
{| | |||
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|min 0 || pour agarose | |||
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|min 20 || remove agarose, place on square coverslips face up | |||
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|min 21 || place cells on 6 slides | |||
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|min 22 || place agarose & slip on G1.1, G1.2, | |||
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|min 26 || place agarose & slip on G5.1, G5.2 | |||
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|min 31 || place agarose & slip on G10.1, G10.2 | |||
# place on agarose: (try this after if time allows) | # place on agarose: (try this after if time allows) |
Revision as of 10:05, 24 February 2010
Cluzel Lab Notebook Daniel Goodman |
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Redo glass experiment from 2/22Prep
Method
Cells
Varying
Deposition Protocol
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