User:Daniel Goodman/Notebook/Cluzel/2010/03/05: Difference between revisions
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== | ==Grow cells== | ||
* | * Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM) | ||
==Made agarose== | |||
* Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM) | |||
==Make chip impression in solidified gel== | |||
* Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down? | |||
''Results: no features found with significant 'push' on 3% gel.'' | |||
==Try transferring again (w/ features)== | |||
*Scope access at 4PM | |||
*Start gel pouring at 2:30 | |||
*Try w/ 3% and 5% agarose | |||
==While waiting...== | |||
*Play with a few featureless gels, practice flipping & transferring | |||
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Revision as of 12:05, 5 March 2010
 Cluzel Lab Notebook Daniel Goodman |
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Grow cells
Made agarose
Make chip impression in solidified gel
Results: no features found with significant 'push' on 3% gel. Try transferring again (w/ features)
While waiting...
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