User:Daniel Goodman/Notebook/Cluzel/2010/03/05
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| - | == | + | ==Grow cells== |
| - | * | + | * Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM) |
| + | ==Made agarose== | ||
| + | * Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM) | ||
| + | |||
| + | ==Make chip impression in solidified gel== | ||
| + | * Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down? | ||
| + | |||
| + | ''Results: no features found with significant 'push' on 3% gel.'' | ||
| + | |||
| + | ==Try transferring again (w/ features)== | ||
| + | *Scope access at 4PM | ||
| + | *Start gel pouring at 2:30 | ||
| + | *Try w/ 3% and 5% agarose | ||
| + | |||
| + | ==While waiting...== | ||
| + | *Play with a few featureless gels, practice flipping & transferring | ||
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Revision as of 15:05, 5 March 2010
 Cluzel Lab Notebook Daniel Goodman |  Main project page Previous entry Next entry
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Grow cells
Made agarose
Make chip impression in solidified gel
Results: no features found with significant 'push' on 3% gel. Try transferring again (w/ features)
While waiting...
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