User:Daniel Goodman/Notebook/Cluzel/2010/03/05

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/03/05 Entry for User:Daniel_Goodman/Notebook/Cluzel)
(Entry title)
Line 1: Line 1:
{{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}}
{{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}}
-
==Entry title==
+
==Grow cells==
-
* Insert content here...
+
* Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)
 +
==Made agarose==
 +
* Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)
 +
 +
==Make chip impression in solidified gel==
 +
* Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?
 +
 +
''Results: no features found with significant 'push' on 3% gel.''
 +
 +
==Try transferring again (w/ features)==
 +
*Scope access at 4PM
 +
*Start gel pouring at 2:30
 +
*Try w/ 3% and 5% agarose
 +
 +
==While waiting...==
 +
*Play with a few featureless gels, practice flipping & transferring
|}
|}
__NOTOC__
__NOTOC__

Revision as of 15:05, 5 March 2010


Cluzel Lab Notebook
Daniel Goodman
Main project page
Previous entry      Next entry

Search this Project

Grow cells

  • Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)

Made agarose

  • Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)

Make chip impression in solidified gel

  • Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?

Results: no features found with significant 'push' on 3% gel.

Try transferring again (w/ features)

  • Scope access at 4PM
  • Start gel pouring at 2:30
  • Try w/ 3% and 5% agarose

While waiting...

  • Play with a few featureless gels, practice flipping & transferring


Personal tools