User:Daniel Goodman/Notebook/Cluzel/2010/03/29
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| - | == | + | ==Prepare for Afternoon/Evening== |
| - | + | ||
| + | ===Agarose=== | ||
| + | 4% agarose, 4.5 ml, 0.18 mg low melt agarose | ||
| + | heat at 80 degrees | ||
| + | (put in heat bath at 1:00) | ||
| + | ===Cells=== | ||
| + | Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) | ||
| + | ===Chips=== | ||
| + | Clean chips in hot water DI bath, 80 degrees | ||
| + | (put in heat bath at 1:00) | ||
| + | ===Strains=== | ||
| + | Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight | ||
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__NOTOC__ | __NOTOC__ | ||
Current revision
 Cluzel Lab Notebook Daniel Goodman |  Main project page Previous entry Next entry
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Prepare for Afternoon/EveningAgarose4% agarose, 4.5 ml, 0.18 mg low melt agarose heat at 80 degrees (put in heat bath at 1:00) CellsPut one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm) ChipsClean chips in hot water DI bath, 80 degrees (put in heat bath at 1:00) StrainsAliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight | ||
