User:Daniel Goodman/Notebook/Cluzel/2010/04/02

From OpenWetWare

< User:Daniel Goodman | Notebook | Cluzel | 2010 | 04(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/04/02 Entry for User:Daniel_Goodman/Notebook/Cluzel)
Current revision (12:22, 2 April 2010) (view source)
(Entry title)
 
Line 1: Line 1:
{{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}}
{{User:Daniel_Goodman/Notebook/Cluzel/Template:Entry_Base}}
-
==Entry title==
+
==Printing spots on glass slide using micrometer stage==
-
* Insert content here...
+
* printed from 10.5-11.1 Y axis
 +
* printing produces reproducible spots, smaller than chip width (need to compute exact diameter)
 +
* printing from higher up might reduce spot size, need to check
 +
* printing repeatedly might reduce spot size
 +
* need to quantify OD:cells per area ratio (might change with stationary vs exponential
 +
* phase and 1 gfp image saved in 4-2 dir on titan
 +
* will transfer to agarose later today: saved slide in petri dish at 12:15 pm
 +
 
 +
==Agarose==
 +
* made 1 ml 4% agarose at 12:20 pm
 +
 
 +
==Cells==
 +
* grew 1 ml cells with 1 col gfp cells (around 11:45 am); forgot to add amp (1 ul), did so at 12:22 pm

Current revision


Cluzel Lab Notebook
Daniel Goodman
Main project page
Previous entry      Next entry

Search this Project

Printing spots on glass slide using micrometer stage

  • printed from 10.5-11.1 Y axis
  • printing produces reproducible spots, smaller than chip width (need to compute exact diameter)
  • printing from higher up might reduce spot size, need to check
  • printing repeatedly might reduce spot size
  • need to quantify OD:cells per area ratio (might change with stationary vs exponential
  • phase and 1 gfp image saved in 4-2 dir on titan
  • will transfer to agarose later today: saved slide in petri dish at 12:15 pm

Agarose

  • made 1 ml 4% agarose at 12:20 pm

Cells

  • grew 1 ml cells with 1 col gfp cells (around 11:45 am); forgot to add amp (1 ul), did so at 12:22 pm



Personal tools