User:Daniel Goodman/Notebook/Cluzel/2010/04/05: Difference between revisions
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==Prepping Plasmid for Electroporation== | ==Prepping Plasmid for Electroporation== | ||
===PZE-12-CFP vector from JM Kim=== | |||
* Vector Nomenclature: http://www.expressys.com/main_vectors.html | |||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
|- | |- | ||
! ng | ! ng/ul | ||
! 260/280 | ! 260/280 | ||
! 260/230 | ! 260/230 | ||
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|} | |} | ||
Per electroporation, need up to 25 ng of DNA | |||
Doing 4 electroporations, in order to ensure competent cells made last week work correctly. | |||
{| class="wikitable" border="1" | |||
|- | |||
! Media | |||
! Plasmid | |||
! Cells | |||
! Why | |||
|- | |||
| LA/Amp | |||
| PZE-12-CFP | |||
| My competent MG1655 | |||
| To get comp cells w/ plasmid | |||
|- | |||
| LA | |||
| PZE-12-CFP | |||
| My competent MG1655 | |||
| To check that my competent cells are alive | |||
|- | |||
| LA/AMP | |||
| Amp Plasmid (from Jeff) | |||
| My competent MG1655 | |||
| To check that my competent cells transform | |||
|- | |||
| LA/AMP | |||
| PZE-12-CFP | |||
| Jeff's competent MG1655 | |||
| To check that my plasmid works/is correct | |||
|} | |||
|} | |} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 09:58, 5 April 2010
 Cluzel Lab Notebook Daniel Goodman |
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Prepping Plasmid for ElectroporationPZE-12-CFP vector from JM Kim
Per electroporation, need up to 25 ng of DNA Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
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