User:Daniel Goodman/Notebook/Cluzel/2010/04/05: Difference between revisions
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Doing 4 electroporations, in order to ensure competent cells made last week work correctly. | Doing 4 electroporations, in order to ensure competent cells made last week work correctly. | ||
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Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book | |||
*need GYT medium | |||
*need SOC medium (1 ml per reaction) | |||
* 10 ng of DNA per experiment | |||
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Revision as of 10:03, 5 April 2010
Cluzel Lab Notebook Daniel Goodman |
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Prepping Plasmid for ElectroporationPZE-12-CFP vector from JM Kim
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
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