User:Daniel Goodman/Notebook/Cluzel/2010/04/05

Cluzel Lab Notebook Daniel Goodman

Main project page Previous entry      Next entry<sitesearch>title=Search this Project</sitesearch>

Prepping Plasmid for Electroporation PZE-12-CFP vector from JM Kim Vector Nomenclature: http://www.expressys.com/main_vectors.html ng/ul 260/280 260/230 56.3 ng 1.92 1.62 Doing 4 electroporations, in order to ensure competent cells made last week work correctly. Media Plasmid Cells Why LA/Amp PZE-12-CFP My competent MG1655 To get comp cells w/ plasmid LA PZE-12-CFP My competent MG1655 To check that my competent cells are alive Spec/AMP PZS4-ATTP My competent MG1655 To check that my competent cells transform LA/AMP PZE-12-CFP Jeff's competent MG1655 To check that my plasmid works/is correct Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book need GYT medium need SOC medium (1 ml per reaction) 10 ng of DNA per experiment