User:Daniel Goodman/Notebook/Weiss/2010/01/19: Difference between revisions
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== | ==Restriction and Gel of PhyB917 & PIF3== | ||
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Samples | |||
First 3 PhyB917 Samples (37 deg C, 10 min) | |||
PIF 3 Sample (65 deg C, 10 min) | |||
Made 75 ml agar gel: | |||
* 75ml TAE | |||
* .75 g Agarose | |||
* 7.5 ul SYBR-SAFE | |||
Put SYBR after cooling, cooled in water bath, set gel ~20 min. | |||
Loading Gel: | |||
{| | |||
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! Lane || Sample || Amt || Loading | |||
|- | |||
| 1 || Hyperladder 1 || 5 ul || n/a | |||
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| 2 || PIF3 || ~15ul || 1.5ul | |||
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| 3-5 || PhyB917 Samp 1-3 || ~15ul || 1.5ul | |||
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Revision as of 11:48, 19 January 2010
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Restriction and Gel of PhyB917 & PIF3Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min) Made 75 ml agar gel:
Put SYBR after cooling, cooled in water bath, set gel ~20 min. Loading Gel:
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