User:Daniel Goodman/Notebook/Weiss/2010/01/19: Difference between revisions

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Put SYBR after cooling, cooled in water bath, set gel ~20 min.
Put SYBR after cooling, cooled in water bath, set gel ~20 min.


==Loading Gel:
=Loading Gel:=
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==Expected for PhyB917:
=Expected for PhyB917:=
Cut with BstBI - two bands, 3579 and 5314  
Cut with BstBI - two bands, 3579 and 5314  


==Expected for PIF3:
=Expected for PIF3:=
Cut with AvrII - two bands, 2164 and 4791  
Cut with AvrII - two bands, 2164 and 4791  


Revision as of 11:49, 19 January 2010

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Restriction and Gel of PhyB917 & PIF3

Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min)

Made 75 ml agar gel:

  • 75ml TAE
  • .75 g Agarose
  • 7.5 ul SYBR-SAFE

Put SYBR after cooling, cooled in water bath, set gel ~20 min.

Loading Gel:

Lane Sample Amt Loading
1 Hyperladder 1 5 ul n/a
2 PIF3 ~15ul 1.5ul
3-5 PhyB917 Samp 1-3 ~15ul 1.5ul

Expected for PhyB917:

Cut with BstBI - two bands, 3579 and 5314

Expected for PIF3:

Cut with AvrII - two bands, 2164 and 4791




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