User:Daniel Goodman/Notebook/Weiss/2010/01/19: Difference between revisions
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Put SYBR after cooling, cooled in water bath, set gel ~20 min. | Put SYBR after cooling, cooled in water bath, set gel ~20 min. | ||
''Note: forgot to swirl after adding sybr, though color looked uniform'' | ''Note: forgot to swirl after adding sybr, though color looked uniform'' | ||
Revision as of 11:51, 19 January 2010
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||||||||
Restriction and Gel of PhyB917 & PIF3Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min) Made 75 ml agar gel:
Put SYBR after cooling, cooled in water bath, set gel ~20 min. Note: forgot to swirl after adding sybr, though color looked uniform
Loading Gel:
Expected for PhyB917:Cut with BstBI - two bands, 3579 and 5314 Expected for PIF3:Cut with AvrII - two bands, 2164 and 4791
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