User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/08/08: Difference between revisions
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==χρόνος πέρασμα August 8th 2011== | ==χρόνος πέρασμα August 8th 2011== | ||
====HydA CAI Optimization Control==== | ====HydA CAI Optimization Control==== | ||
=====ABSTRACT===== | |||
* Design of the primers for the amplification of the optimized and wild-type HydA. | |||
== == | |||
* Today I designed the primers that will be needed to amplify the CDS of the optimized and wild-type HydA genes. | * Today I designed the primers that will be needed to amplify the CDS of the optimized and wild-type HydA genes. | ||
Revision as of 03:55, 25 September 2011
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χρόνος πέρασμα August 8th 2011HydA CAI Optimization ControlABSTRACT
HYDA WILD-TYPE NON OPTIMIZED NheI site - HydA 5' end first 25 nt (34 nt) Tm = 60°C CTAGCTAGCATGAAAACAATAATCTTAAATGGCA HydA 3' end last 20 nt - Double Stop Codon - Suffix (61 nt) AAAATGTTTCAAAACATGAATAATAATACTAGTAGCGGCCGCTGCAGAACCAATGCATTGG HydA 3' end last 20 nt - Double Stop Codon - Suffix - reverse complement (61 nt) Tm = 71°C CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTATTCATGTTTTGAAACATTTT
NheI site - HydA 5' end first 25 nt (34 nt) Tm = 67°C CTAGCTAGCATGAAGACCATCATCCTGAACGGCA HydA 3' end last 20 nt - Double Stop Codon - Suffix (61 nt) AGAACGTCTCGAAGCATGAGTAATAATACTAGTAGCGGCCGCTGCAGAACCAATGCATTGG HydA 3' end last 20 nt - Double Stop Codon - Suffix - reverse complement (61 nt) Tm = 74°C CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTACTCATGCTTCGAGACGTTCT
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