User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> UNAM Genomics Mexico 2011</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==χρόνος πέρασμα September 11th 2011==
* Insert content here...
====HydA CAI Optimization Control====
* Today, I repeated the PCR that is supposed to amplify the optimized HydA CDS that will serve to prove if the codon optimization that was performed on the sequences we sent synthesize did make a difference for Rhizobium etli CFN42 in its growth. As the past PCR [INSERTAR LINK] was not succesful, I now tried to amplify from another stock of the plasmid, one that was cutted with restriction enzymes (is linearized), extracted from an E.coli transformation. I used Taq platinum from Invitrogen.




Revision as of 02:36, 25 September 2011

UNAM Genomics Mexico 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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χρόνος πέρασμα September 11th 2011

HydA CAI Optimization Control

  • Today, I repeated the PCR that is supposed to amplify the optimized HydA CDS that will serve to prove if the codon optimization that was performed on the sequences we sent synthesize did make a difference for Rhizobium etli CFN42 in its growth. As the past PCR [INSERTAR LINK] was not succesful, I now tried to amplify from another stock of the plasmid, one that was cutted with restriction enzymes (is linearized), extracted from an E.coli transformation. I used Taq platinum from Invitrogen.



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