User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/20: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
Line 48: | Line 48: | ||
|<strong>50μl</strong> | |<strong>50μl</strong> | ||
|} | |} | ||
* Incubation 5 minutes at 94°C | |||
* 30 Cycles | |||
** Denature: 94°C for 15 seconds | |||
** Anneal: 55°C-68°C for 30 seconds | |||
** Extend: 68°C for 1:55 minutes | |||
* The reactions were maintained at 4°C once the cycles finished. | |||
* Once finished the PCR, I ran a 1% agarose electrophoretic gel. 130 V 35 minutes, 5μl from each PCR tube were used per each well. | |||
Line 77: | Line 88: | ||
|-- | |-- | ||
|} | |} | ||
[[Image:dramirez_PCR_Pfxplatinum_temperaturegradient_HydA.jpg|320px|center]] | |||
Revision as of 01:49, 25 September 2011
UNAM Genomics Mexico 2011 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||
χρόνος πέρασμα September 20th 2011HydA CAI Optimization Control
|