User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/24: Difference between revisions
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==== HydA CAI Optimization Control==== | ==== HydA CAI Optimization Control==== | ||
=====ABSTRACT===== | =====ABSTRACT===== | ||
* Electrophoretic gels from the last PCRs I did [[User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/22|this]]. I tried concentrating the products but nothing appeared on the gels. | * Electrophoretic gels from the last PCRs I did [[User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/22|this]] and [[User:Daniel Ramirez/Notebook/UNAM Genomics Mexico 2011/2011/09/21| this]]. I tried concentrating the products but nothing appeared on the gels. | ||
== == | == == | ||
* Today I concentrated all the PCR products to see if once concentrated I could see any amplification (just as Paulina did [[User:Paulina_Alatriste/Notebook/UNAM_Genomics_Mexico_2011/2011/09/04|here]]). The 12 PCR tubes from the PCR using Pfx Platinum Polymerase with the PCRx Enhancer Buffer at different concentrations as well as the 12 PCR tubes from the PCR using Taq Polymerase with a combination of primers were left into the SpeedVac for approximately 30 minutes at high temperature. Once most of the solvent got evaporated, I added 20 μl of HPLC water and left the tubes at 42°C during 20 minutes to dissolve the contents. I proceeded to run the samples into a 1% electrophoretic gel (130 V 35 m). 5 μl from the total of 20 μl from each reaction was used. | * Today I concentrated all the PCR products to see if once concentrated I could see any amplification (just as Paulina did [[User:Paulina_Alatriste/Notebook/UNAM_Genomics_Mexico_2011/2011/09/04|here]]). The 12 PCR tubes from the PCR using Pfx Platinum Polymerase with the PCRx Enhancer Buffer at different concentrations as well as the 12 PCR tubes from the PCR using Taq Polymerase with a combination of primers were left into the SpeedVac for approximately 30 minutes at high temperature. Once most of the solvent got evaporated, I added 20 μl of HPLC water and left the tubes at 42°C during 20 minutes to dissolve the contents. I proceeded to run the samples into a 1% electrophoretic gel (130 V 35 m). 5 μl from the total of 20 μl from each reaction was used. |
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χρόνος πέρασμα September 24th 2011HydA CAI Optimization ControlABSTRACT
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