User:Darrell A Humphries Jr/Notebook/Biology 210 at AU

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The state of the Hay Infusion Culture me and my lab partners observed, show notable physical change from the first time the Hay Infusion Culture were observed. A stronger, darker odor is emitting from its container (sewer-like), while evidence of more bacteria growth have become present. The turbidity of the water has increased, it has more difficult to see through the water as it is much more 'cloudy' than before, possibly due to an increase in particles and bacteria.

Dilution |Agar Type | # Colonies on plate | Conversion Factor | Colonies/mL |

10^-3 |nutrient | 756 | x10^3 | 756,000 |

10^-5 |nutrient | 64 | x10^5 | 6,400,000 |

10^-7 |nutrient | 2 | x10^7 | 20,000,000 |

10^-9 |nutrient | 0 | x10^9 | 0 |

10^-3 |nutrient + tet | 70 | x10^3 | 70,000 |

10^-5 |nutrient + tet | 0 | x10^5 | 0 |

10^-7 |nutrient + tet | 0 | x10^7 | 0 |

10^-9 |nutrient + tet | 0 | x10^9 | 0 |

  • This table indicates that the antibiotic Tetracycline was effective with inhibiting bacteria growth on the Agar Plates; so effective that our last three(3) agar plates recorded zero(0) bacteria colonies on the Agar Plate. Dilution 10^-7 without the tetracycline recorded 20 million colonies/mL of bacteria, while dilution 10^-7 with tetracycline recorded zero(0) colonies/mL of bacteria.

Mechanisms of action for tetracycline:Citation -> Antimicrobial Research Centre and Division of Microbiology, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom,1 and Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195-72382 <- \Mechanisms of action for tetracycline/

  • inhibit protein synthesis by preventing the attachment of aminoacyl-tRNA to the ribosomal acceptor (A) site

Types of Bacteria effected:

  • Tetracyclines are broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria, atypical organisms such as chlamydiae, mycoplasmas, and rickettsiae, and protozoan parasites.

Colony Label | Plate Type| Colony Description + Cell Description | Gram + or Gram - | Additional Notes | 

    1       |     -     |        Orange, white like              |                  |
                            pigments. Circular & irregular.      |      negative    |
                            Flat & raised elevation. Filamentous |                  |
                                     Colony.                     |                  |

    2       |     -     |  Yellow, orange, white, smooth,        |                  |
                           some convex elevation. Rhizoid        |   positive       |  
                              colony and circular                |                  |

    3       |     +     | Mixture of circular, irregular         |                  |  
                         & filamintous colonies. Lobat &         |    positive      |  gram positive 1um- pink circular bacteria 
                           entire edge. Convex elevation,        |                  |
                          smooth surface, jell-like.             |                  |

    4       |     +     |       No Bacteria Growth               |     negative     |


Media:https://scontent-lga3-1.xx.fbcdn.net/hphotos-xft1/v/t34.0-12/12654522_10200225055808888_4943828854081192355_n.jpg?oh=c7452ace4a24d4962cb0c40a68df8b14&oe=56B578F3 Here in this image of colony label 3,

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