User:DavidDrubin

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back to [http://www.openwetware.org/index.php?title=Silver_Lab Silver Lab Main Page]
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back to [http://www.openwetware.org/index.php?title=Silver:_Lab_Members Silver Lab Members]
== Biographical Info ==
== Biographical Info ==
[[Image:drubin.jpg]]
[[Image:drubin.jpg]]
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david_drubin at hms dot harvard dot edu
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-4th year Postdoctoral Research Fellow
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-2nd year Postdoctoral Research Fellow
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-Ph.D. Integrative Biosciences-Molecular Medicine, Penn State University College of Medicine, 2004
-Ph.D. Integrative Biosciences-Molecular Medicine, Penn State University College of Medicine, 2004
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== Research Interests ==
== Research Interests ==
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The relationship of transcriptional activity with the intra-nuclear positioning of genes has been well documented, particularly relative to the periphery of the nucleusLocalization at the nuclear periphery is traditionally a hallmark of gene silencingHowever, our lab and others using the yeast ''Saccharomyces cerevisiae'' as a model, have shown that active genes are also present at the nuclear periphery and that the nuclear pore complex is an important mediator of genome organization. In fact, certain genes are recruited to the NPC upon transcriptional induction. 
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We are pursuing the design and construction of a mammalian cell-based sensor-memory device.  Such a device will be able to report whether specific events occur in a cell or whether certain compounds are present in the cell's environment, and then store this information in itself and in all the cell's progenyFurthermore, such a system could also be designed to report on quantity of a particular stimulusA key feature of such a system will be its portability to various environmental stimuli or specific cellular events, and will not be limited to very specific chemical inductions.  We are also interested in the applied uses of a memory device in more functionally creative and complex cellular circuits.
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My research interests involve the mechanism(s) of genome interaction with the nuclear pore complex and nuclear periphery, especially in regards to gene activation and the dynamics of the process.  Chromosomes are not static structures, and their constrained diffusive movement most certainly plays a role in many nuclear processes. We have employed the lac operator/GFP-lac repressor system to visualize the real-time motion of the ''GAL'' locus in live-cells.  In this way we can observe how transcriptional induction can affect ''GAL'' locus motion in a population of yeastFurthermore, we can then perturb the system via mutants, etc. to begin looking at what factors play a role in the phenomenon of gene recruitment to the nuclear periphery.
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= Publications =
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Ajo-Franklin CM*, Drubin DA*, Eskin JA, Gee EPS, Landgraf D, Phillips I, and Silver PA.   Rational design of memory in eukaryotic cells.  ''Genes Dev '' 21: 2271-2276*authors contributed equally to this work.
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Drubin, DA, Way, JC, and Silver, PA.  (2007).  Designing biological systems.  ''Genes Dev  ''  21(3): 242-254.
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== Publications ==
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Drubin, DA, Garakani, AM, and Silver, PA.  (2006).  Motion as a phenotype:  the use of live-cell imaging and machine visual screening to characterize transcription-dependent chromosome dynamics.  ''BMC Cell Biology'' 7: 19.
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Drubin, DA and Clawson, GA (2004). Spontaneous transformation of an immortalized hepatocyte cell line: potential role of a nuclear protease.  ''Cancer Letters''   213: 39-48.
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Dhamne C, Drubin DA, Duncan K, Tevethia MJ, Clawson GA. (2006) The chloromethylketone protease inhibitor AAPF(CMK) also targets ATP-dependent helicases and SAP-domain proteins.  ''J Cell Biochem'' 100: 716-726.
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Drubin, DA, Smith, JS, Liu, W, Zhao, W, Chase, GA, and Clawson, GA.  (2005).  Comparison of Cryopreservation and Standard Needle Biopsy for Gene Expression Profiling of Human Breast Cancer Specimens. ''Breast Cancer Research and Treatment''   90: 93-96.  
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Drubin, DA*, McLaughlin-Drubin, ME*, Clawson, GA, and Meyers, C.  (2006).  A Protease Inhibitor Specifically Inhibits Growth of HPV Infected Tissue. ''Molecular Therapy'' 13(6): 1142-8.  *authors equally contributed to this work.
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Drubin, DA*, McLaughlin-Drubin, ME*, Clawson, GA, and Meyers, CA Protease Inhibitor Specifically Inhibits Growth of HPV Infected TissueManuscript accepted, ''Molecular Therapy''.  *authors equally contributed to this work.
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Casolari, JM, Brown, CR, Drubin, DA, Rando, OJ and Silver, PA(2005).  Developmentally Induced Changes in Transcriptional Program Alter Spatial Organization Across Chromosomes.  ''Genes and Development''   19: 1188-1198.   
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Casolari, JM, Brown, CR, Drubin, DA, Rando, OJ and Silver, PA.  (2005).  Developmentally Induced Changes in Transcriptional Program Alter Spatial Organization Across Chromosomes.  ''Genes and Development''  19: 1188-1198.
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Drubin, DA, Smith, JS, Liu, W, Zhao, W, Chase, GA, and Clawson, GA.  (2005).  Comparison of Cryopreservation and Standard Needle Biopsy for Gene Expression Profiling of Human Breast Cancer Specimens.  ''Breast Cancer Research and Treatment''  90: 93-96.   
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G. Clawson, D. Drubin, M. McLaughlin-Drubin, C. Meyers“A Nuclear Protease Inhibitor as a Topical Therapeutic for Cervical Dysplasias”, patent pending.
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Drubin, DA and Clawson, GA (2004). Spontaneous transformation of an immortalized hepatocyte cell line: potential role of a nuclear protease.  ''Cancer Letters''  213: 39-48.
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== Interests & Hobbies ==
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G. Clawson, D. Drubin, M. McLaughlin-Drubin, C. Meyers.  “Compositions and methods for inhibiting abnormal cell growth”, patent pending.

Current revision

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Biographical Info

Image:drubin.jpg david_drubin at hms dot harvard dot edu

-4th year Postdoctoral Research Fellow

-Ph.D. Integrative Biosciences-Molecular Medicine, Penn State University College of Medicine, 2004

    Graduate Advisor:  Dr. Gary A. Clawson, Clawson lab, Jake Gittlen Cancer Research Institute
    Research Topic:  Early changes in carcinogenesis

-B.S. Biochemistry and Molecular Biology, University of Massachusetts at Amherst, 1997

    Honors Advisor:  Dr. Maurille "Skip" J. Fournier
    Research Topic:  snoRNA biogenesis

Research Interests

We are pursuing the design and construction of a mammalian cell-based sensor-memory device. Such a device will be able to report whether specific events occur in a cell or whether certain compounds are present in the cell's environment, and then store this information in itself and in all the cell's progeny. Furthermore, such a system could also be designed to report on quantity of a particular stimulus. A key feature of such a system will be its portability to various environmental stimuli or specific cellular events, and will not be limited to very specific chemical inductions. We are also interested in the applied uses of a memory device in more functionally creative and complex cellular circuits.

Publications

Ajo-Franklin CM*, Drubin DA*, Eskin JA, Gee EPS, Landgraf D, Phillips I, and Silver PA. Rational design of memory in eukaryotic cells. Genes Dev 21: 2271-2276. *authors contributed equally to this work.

Drubin, DA, Way, JC, and Silver, PA. (2007). Designing biological systems. Genes Dev 21(3): 242-254.

Drubin, DA, Garakani, AM, and Silver, PA. (2006). Motion as a phenotype: the use of live-cell imaging and machine visual screening to characterize transcription-dependent chromosome dynamics. BMC Cell Biology 7: 19.

Dhamne C, Drubin DA, Duncan K, Tevethia MJ, Clawson GA. (2006) The chloromethylketone protease inhibitor AAPF(CMK) also targets ATP-dependent helicases and SAP-domain proteins. J Cell Biochem 100: 716-726.

Drubin, DA*, McLaughlin-Drubin, ME*, Clawson, GA, and Meyers, C. (2006). A Protease Inhibitor Specifically Inhibits Growth of HPV Infected Tissue. Molecular Therapy 13(6): 1142-8. *authors equally contributed to this work.

Casolari, JM, Brown, CR, Drubin, DA, Rando, OJ and Silver, PA. (2005). Developmentally Induced Changes in Transcriptional Program Alter Spatial Organization Across Chromosomes. Genes and Development 19: 1188-1198.

Drubin, DA, Smith, JS, Liu, W, Zhao, W, Chase, GA, and Clawson, GA. (2005). Comparison of Cryopreservation and Standard Needle Biopsy for Gene Expression Profiling of Human Breast Cancer Specimens. Breast Cancer Research and Treatment 90: 93-96.

Drubin, DA and Clawson, GA (2004). Spontaneous transformation of an immortalized hepatocyte cell line: potential role of a nuclear protease. Cancer Letters 213: 39-48.

G. Clawson, D. Drubin, M. McLaughlin-Drubin, C. Meyers. “Compositions and methods for inhibiting abnormal cell growth”, patent pending.

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