User:DavidRamos/Lab/2006-6-15

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June 15th

Morning

  • Brainstorming with Pam
  • Conduct ligation reaction
    • 6uL insert, 2uL vector, 2uL buffer, 10uL other buffer
    • 5 min incubation @ RT
  • Conduct transformation
    • 20mL cells for positive, negative, and exp. ea (top 10)

Afternoon

  • Plate transformant cells on LB-CARB
  • Brainstorming session in the afternoon
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