User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25
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Current revision (14:09, 30 January 2013) (view source) (→Preparation of a dilution sequence) |
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==Preparation of a dilution sequence== | ==Preparation of a dilution sequence== | ||
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | ||
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Cell line: HEK 293-Luc14 | Cell line: HEK 293-Luc14 | ||
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Plates: 6-well plates | Plates: 6-well plates | ||
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Dilution rate: 1:5 | Dilution rate: 1:5 | ||
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Starting with a full grown plate mixed into 10ml liquid. | Starting with a full grown plate mixed into 10ml liquid. | ||
Well No. : Approximate avg. cell count | Well No. : Approximate avg. cell count | ||
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1: 1,000,000 | 1: 1,000,000 | ||
2: 200,000 | 2: 200,000 | ||
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3: 40,000 | 3: 40,000 | ||
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4: 8,000 | 4: 8,000 | ||
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5: 1,600 | 5: 1,600 | ||
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6: 320 | 6: 320 | ||
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7: 64 | 7: 64 | ||
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8: ~12 | 8: ~12 | ||
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9: ~2 | 9: ~2 | ||
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10:<1 | 10:<1 | ||
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Preparation of a dilution sequenceTo determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid. Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1 | |
