User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Preparation of a dilution sequence== | ==Preparation of a dilution sequence== | ||
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | ||
Cell line: HEK 293-Luc14 | Cell line: HEK 293-Luc14 | ||
Plates: 6-well plates | Plates: 6-well plates | ||
Dilution rate: 1:5 | Dilution rate: 1:5 | ||
Starting with a full grown plate mixed into 10ml liquid. | Starting with a full grown plate mixed into 10ml liquid. | ||
Well No. : Approximate avg. cell count | Well No. : Approximate avg. cell count | ||
1: 1,000,000 | 1: 1,000,000 | ||
2: 200,000 | 2: 200,000 | ||
3: 40,000 | 3: 40,000 | ||
4: 8,000 | 4: 8,000 | ||
5: 1,600 | 5: 1,600 | ||
6: 320 | 6: 320 | ||
7: 64 | 7: 64 | ||
8: ~12 | 8: ~12 | ||
9: ~2 | 9: ~2 | ||
10:<1 | 10:<1 | ||
Latest revision as of 22:23, 26 September 2017
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Preparation of a dilution sequenceTo determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid. Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1 | |
