User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25
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| - | == | + | ==Preparation of a dilution sequence== |
| - | + | To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | |
| + | Cell line: HEK 293-Luc14 | ||
| + | Plates: 6-well plates | ||
| + | Dilution rate: 1:5 | ||
| + | Starting with a full grown plate mixed into 10ml liquid. | ||
| + | Well No. : Approximate avg. cell count | ||
| + | 1: 1,000,000 | ||
| + | 2: 200,000 | ||
| + | 3: 40,000 | ||
| + | 4: 8,000 | ||
| + | 5: 1,600 | ||
| + | 6: 320 | ||
| + | 7: 64 | ||
| + | 8: ~12 | ||
| + | 9: ~2 | ||
| + | 10:<1 | ||
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Revision as of 14:08, 30 January 2013
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Preparation of a dilution sequenceTo determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid. Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1 | |
