User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/01/25 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
 
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Preparation of a dilution sequence==
* Insert content here...
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.


Cell line: HEK 293-Luc14
Plates: 6-well plates
Dilution rate: 1:5
Starting with a full grown plate mixed into 10ml liquid.
Well No. : Approximate avg. cell count
1:  1,000,000
2:  200,000
3:  40,000
4:  8,000
5:  1,600
6:  320
7:  64
8:  ~12
9:  ~2
10:<1


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 22:23, 26 September 2017

Project name Main project page
Next entry

Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.

Cell line: HEK 293-Luc14

Plates: 6-well plates

Dilution rate: 1:5

Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count

1: 1,000,000

2: 200,000

3: 40,000

4: 8,000

5: 1,600

6: 320

7: 64

8: ~12

9: ~2

10:<1


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern/2013/01/25&oldid=1011840"