User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25: Difference between revisions
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== | ==Preparation of a dilution sequence== | ||
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. | |||
Cell line: HEK 293-Luc14 | |||
Plates: 6-well plates | |||
Dilution rate: 1:5 | |||
Starting with a full grown plate mixed into 10ml liquid. | |||
Well No. : Approximate avg. cell count | |||
1: 1,000,000 | |||
2: 200,000 | |||
3: 40,000 | |||
4: 8,000 | |||
5: 1,600 | |||
6: 320 | |||
7: 64 | |||
8: ~12 | |||
9: ~2 | |||
10:<1 | |||
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Revision as of 11:08, 30 January 2013
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Preparation of a dilution sequenceTo determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid. Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1 | |
