User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/28
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First observation on cell growth for dilution sequenceWell No.(approx. avg. cell count): Obervations 1(1e6): Continuos structure with a reticular structure base on star like extensions of the cells. No distinct colonies. Close to the edge of the well, but not exactly at the edge the cells form a very dense ring enclosing the whole plate. 2(2e5): Continuos structure with a reticular structure base on star like extensions of the cells. No distinct colonies. Less dene then well number one. Same ring structure with high cell density. 3(4e4): Uniformly distributed individual cells, only few connections between cells. No distinct colonies. More dense ring structure close to the edge, these cells have a lot of connections. 4(8e3): Uniformly distributed individual cells, far apart, no connections. Some distinct cell clusters with <10 cells. 5(1.6e3): Uniformly distributed individual cells, far apart, no connections. Only very few visible clusters of cells with <10 cells. 6(320): Uniformly distributed individual cells, far apart, no connections. No visible clusters. 7(64): No visible cell found. 8(12): No visible cell found. 9(~2): No visible cell found. 10(<1): No visible cell found. | |
