User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|

Latest revision as of 22:25, 26 September 2017

Project name Main project page
Previous entry      Next entry

02/06/13

  • HEK293-luc14 plate: 6-well plate


HEK293-luc14 plate

  • Plated HEK293-luc14 positive control cells at optimal density (1.6x103), as determined by previous dilution plating
    • Tryspinized cells, brought conc. to 1x106/mL
    • Added 0.5 mL to 12 mL medium (1/25 dilution)
    • Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution)
    • Added 1 mL of the 1/625 dilution to each well
    • Added an additional 1 mL growth medium to each well (to prevent the medium from drying out)
  • Grow for 7 days to get colonies
  • Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern/2013/02/06&oldid=1011936"