User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06

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(Autocreate 2013/02/06 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
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==Entry title==
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==02/06/13==
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* HEK293-luc14 plate: 6-well plate
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'''HEK293-luc14 plate'''
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* Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating
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** Tryspinized cells, brought conc. to 1x10<sup>6</up>/mL
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** Added 0.5 mL to 12 mL medium (1/25 dilution)
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** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution)
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** Added 1 mL of the 1/625 dilution to each well
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** Added an additional 1 mL growth medium to each well (to prevent the medium from drying out)
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* Grow for 7 days to get colonies
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* Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives.

Revision as of 21:33, 6 February 2013

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02/06/13

  • HEK293-luc14 plate: 6-well plate



HEK293-luc14 plate

  • Plated HEK293-luc14 positive control cells at optimal density (1.6x103), as determined by previous dilution plating
    • Tryspinized cells, brought conc. to 1x106</up>/mL
    • Added 0.5 mL to 12 mL medium (1/25 dilution)
    • Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution)
    • Added 1 mL of the 1/625 dilution to each well
    • Added an additional 1 mL growth medium to each well (to prevent the medium from drying out)
  • Grow for 7 days to get colonies
  • Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives.


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