User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06: Difference between revisions
From OpenWetWare
(Autocreate 2013/02/06 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==02/06/13== | ||
* | * HEK293-luc14 plate: 6-well plate | ||
---- | |||
'''HEK293-luc14 plate''' | |||
* Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating | |||
** Tryspinized cells, brought conc. to 1x10<sup>6</up>/mL | |||
** Added 0.5 mL to 12 mL medium (1/25 dilution) | |||
** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution) | |||
** Added 1 mL of the 1/625 dilution to each well | |||
** Added an additional 1 mL growth medium to each well (to prevent the medium from drying out) | |||
* Grow for 7 days to get colonies | |||
* Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives. | |||
Revision as of 19:33, 6 February 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
02/06/13
HEK293-luc14 plate
|