User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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# 6-well plate: 2 mL of 1:25 dln., ~80,000 cells/ well | # 6-well plate: 2 mL of 1:25 dln., ~80,000 cells/ well | ||
# 6-well plate: 2 mL of 1:625 dln., ~3,200 cells/ well | # 6-well plate: 2 mL of 1:625 dln., ~3,200 cells/ well | ||
# 96-well plate: 200 uL of 1:40,000 dln., ~5 cells/ well | # 96-well plate: 200 uL of 1:40,000 dln., ~5 cells/ well | ||
Notes: | |||
* Was careful to gently mix stock tube by inverting after every column was filled to ensure that cells stayed suspended in the stock tube. | |||
* Used 20% FBS for 96-well plate to encourage growth of isolated/ low-concentration cells. | |||
Latest revision as of 22:28, 26 September 2017
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02/15/13
HEK293-luc #4 cell culturing, trial 2
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