User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15: Difference between revisions
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* | * HEK293-luc cell culturing, trial 2 | ||
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''HEK293-luc #4 cell culturing, trial 2'' | |||
* Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well | |||
* Retry 6-well plates and also try 96-well plate | |||
* Made three new plates of HEK293-luc #4 cells | |||
* Started with ~1x10<sup>6</sup> harvested cells (confluent T25 flask) | |||
* Made the following dilutions: | |||
**(1) 1:25 dilution of harvested cells = 40,000 cells/mL 10% FBS DMEM | |||
**(2) 1:25 dilution of tube 1 = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM | |||
**(3) 1:100 dilution of 1x10<sup>6 cells/mL | |||
# 6-well plate: 2 mL , 80,000 cells/ well | |||
# 6-well plate: 2 mL 10% FBS DMEM, 3200 cells/ well | |||
Revision as of 18:30, 18 February 2013
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02/15/13
HEK293-luc #4 cell culturing, trial 2
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