User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15: Difference between revisions
From OpenWetWare
Line 27: | Line 27: | ||
# 6-well plate: 2 mL of 1:25 dln., ~80,000 cells/ well | # 6-well plate: 2 mL of 1:25 dln., ~80,000 cells/ well | ||
# 6-well plate: 2 mL of 1:625 dln., ~3,200 cells/ well | # 6-well plate: 2 mL of 1:625 dln., ~3,200 cells/ well | ||
# 96-well plate: 200 uL of 1:40,000 dln., ~5 cells/ well | # 96-well plate: 200 uL of 1:40,000 dln., ~5 cells/ well | ||
Notes: | |||
* Was careful to gently mix stock tube by inverting after every column was filled to ensure that cells stayed suspended in the stock tube. | |||
* Used 20% FBS for 96-well plate to encourage growth of isolated/ low-concentration cells. | |||
Revision as of 18:41, 18 February 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
02/15/13
HEK293-luc #4 cell culturing, trial 2
|