User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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'''DAPI/ luciferase staining'''<br> | '''DAPI/ luciferase staining'''<br> | ||
* D-luciferin stock solution: | * D-luciferin stock solution: 30mg/ml in 1xPBS, working solution is 150ug/ml (200x) | ||
* Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O | * Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O | ||
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* Did not detect D-luc signal. Perhaps D-luc imaging should start immediately after adding reagents. The D-luc signal has a short half-life. | * Did not detect D-luc signal. Perhaps D-luc imaging should start immediately after adding reagents. The D-luc signal has a short half-life. | ||
* Cells rounded up a bit after sitting in PBS. Next time use 10% FBS in PBS, or try DMEM without red dye. | * Cells rounded up a bit after sitting in PBS. Next time use 10% FBS in PBS, or try DMEM without red dye. | ||
* DAPI signal was barely visible | |||
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* HEK293 Gal4-EED, 1:4 | * HEK293 Gal4-EED, 1:4 | ||
* HEK293 Gal4-EED, 1:4 in 0.5 μg/mL puromycin (to select for Gal4-EED RNAi tuner) | * HEK293 Gal4-EED, 1:4 in 0.5 μg/mL puromycin (to select for Gal4-EED RNAi tuner) | ||
Latest revision as of 22:29, 26 September 2017
Project name | Main project page Previous entry Next entry |
02/23/13
DAPI/ luciferase staining
Used 3 wells:
Results/ Conclusions:
New cell plates
Stock cultures (T75 flasks)
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