User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/26: Difference between revisions

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==Entry title==
==02/26/13==
* Insert content here...
HEK293 GAL-4 EED DAPI staining
 
 
----
* DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O
* Diluted Hoescht to working concentration (1x) in 1xPBS.  
* Added 2 mL to each well
* Let sit at room temperature for 5 mins in the dark
 
 
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Results:
* strong fluorescence signal only in dead/detached cells
* very weak signal in living cells
 
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Next steps:
* Testing if longer dwell time prior to imaging improves DAPI signal
* assay the possibility of different dye for nucleus staining in replicating cells
* assay the possibilities to detect luciferase signal




Revision as of 21:32, 18 March 2013

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02/26/13

HEK293 GAL-4 EED DAPI staining


  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Added 2 mL to each well
  • Let sit at room temperature for 5 mins in the dark


Results:

  • strong fluorescence signal only in dead/detached cells
  • very weak signal in living cells

Next steps:

  • Testing if longer dwell time prior to imaging improves DAPI signal
  • assay the possibility of different dye for nucleus staining in replicating cells
  • assay the possibilities to detect luciferase signal



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