User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==02/26/13==
==02/26/13==
GAL-4 EED DAPI staining
HEK293 GAL-4 EED DAPI staining




Revision as of 21:32, 18 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

02/26/13

HEK293 GAL-4 EED DAPI staining


  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Added 2 mL to each well
  • Let sit at room temperature for 5 mins in the dark


Results:

  • strong fluorescence signal only in dead/detached cells
  • very weak signal in living cells

Next steps:

  • Testing if longer dwell time prior to imaging improves DAPI signal
  • assay the possibility of different dye for nucleus staining in replicating cells
  • assay the possibilities to detect luciferase signal



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern/2013/02/26&oldid=683976"