User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/26: Difference between revisions
From OpenWetWare
David Dreher (talk | contribs) (Autocreate 2013/02/26 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern) |
David Dreher (talk | contribs) |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==02/26/13== | ||
* | GAL-4 EED DAPI staining | ||
---- | |||
* DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O | |||
* Diluted Hoescht to working concentration (1x) in 1xPBS. | |||
* Added 2 mL to each well | |||
* Let sit at room temperature for 5 mins in the dark | |||
---- | |||
Results: | |||
* strong fluorescence signal only in dead/detached cells | |||
* very weak signal in living cells | |||
---- | |||
Next steps: | |||
* Testing if longer dwell time prior to imaging improves DAPI signal | |||
* assay the possibility of different dye for nucleus staining in replicating cells | |||
* assay the possibilities to detect luciferase signal | |||
Revision as of 21:28, 18 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
02/26/13GAL-4 EED DAPI staining
Results:
Next steps:
|