User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/26

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(Autocreate 2013/02/26 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
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==Entry title==
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==02/26/13==
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GAL-4 EED DAPI staining
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----
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* DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O
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* Diluted Hoescht to working concentration (1x) in 1xPBS.  
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* Added 2 mL to each well
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* Let sit at room temperature for 5 mins in the dark
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----
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Results:
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* strong fluorescence signal only in dead/detached cells
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* very weak signal in living cells
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----
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Next steps:
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* Testing if longer dwell time prior to imaging improves DAPI signal
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* assay the possibility of different dye for nucleus staining in replicating cells
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* assay the possibilities to detect luciferase signal

Revision as of 23:28, 18 March 2013

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02/26/13

GAL-4 EED DAPI staining



  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Added 2 mL to each well
  • Let sit at room temperature for 5 mins in the dark



Results:

  • strong fluorescence signal only in dead/detached cells
  • very weak signal in living cells

Next steps:

  • Testing if longer dwell time prior to imaging improves DAPI signal
  • assay the possibility of different dye for nucleus staining in replicating cells
  • assay the possibilities to detect luciferase signal



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